Helena Electrophoresis Supplies
- Power
- Chamber
- Blotter
- Buffer
- Stain/Clearing
- Buffer/Stain
- Chamber/Weight
- Sample Application Devices
- REP/REP 3 Accessories
- Miscellaneous Supplies
Electrophoresis Power Supplies
TITAN® Plus Power Supply
| Cat. No. | Item |
| 1504 | TITAN Plus Power Supply, 110V |
| 1505 | TITAN Plus Power Supply, 220V |
TITAN Plus is a completely solid state unit. Digital voltage and current display provides greater accuracy in voltage selection. Synchronous electrophoresis timer assures precise control; timer automatically shuts off current and signals end of run. For use with cellulose acetate, agarose and agar. Recommended for use with Quick Gel Chamber, (Cat. No. 1284).
TITAN Plus Specifications
Voltage: 0-500 VDC. Digital display. Accuracy ± 3V.
Current: Up to 125 mA. Digital display. Accuracy ± 2 mA.
Chamber Requirements: Accepts all standard electrophoresis chambers
Timer: Synchronous, electrical. Up to 60 minutes automatic timing. Audiovisual signal indicates end of run. "Hold" allows for continuous operation beyond 60 minutes.
Safety Feature: Fuse holder: 110V-1A; 220V-.5A, 3 prong plug, grounded chassis
Output Jacks: Two sets labeled three ways: + and -, Red and Black; Positive and Negative. Standard banana jack spacing.
Dimensions: 5" H x 10" W x 9.5" D (12.8 x 25.5 x 24.2 cm)
Weight: 11 lbs. (4.95 kg)
EWS Digital Power Supply
| Cat. No. | Item |
| 1520 | EWS Power Supply, 110V |
| 1521 | EWS Power Supply, 220V |
| 1150 | Comprehensive Replacement Agreement |
The microprocessor-controlled EWS Power Supply provides a digital display showing voltage/current levels with ± 1% accuracy and elapsed time ± second. Timer allows programming in 1 second intervals up tp 99 minutes, 59 seconds. Power output ranges from 50-400 VDC in constant voltage mode in 1.0 V increments; 2-60 mA in constant current mode in 0.1 mA increments. Standard banana plug output. Recommended for use with Laurell Rocket electrophoresis procedures (Cat. Nos. 5357 and 5359).
EWS Specifications
Modes of Operation: Constant voltage or constant current. Time automatically or manually controlled.
Input Power: Constant Voltage Output Range: 50 to 400 VDC ± 1% or ± 1 unit in 1.0V increments. Constant Current Output: 2 to 60 mA ± 1% or ± 1 unit in 0.1 mA increments.
Meter Accuracy: ± 1V or ± 0.1 mA or ± 1%
Ripple (Noise): Less than 1% rms of output amplitude
Timer: Up to 99 min. 59 sec. in 1 sec. increments
AC Fuse (Top): 2 A, 250V
High Voltage Fuse (Bottom): 0.2 A, 250V
Dimensions: 5" H x 10.2" W x 12" D (12.5 x 25.5 x 30 cm)
Weight: 5.5 lbs. (2.5 kg)
Electrophoresis Chambers and Accessories
TITAN GEL® Chamber
| Cat. No. | Item |
| 4063 | TITAN GEL Chamber w/ Heat Shield, 1/pkg |
The TITAN GEL Chamber is for Titan III cellulose acetate, TITAN GEL Agarose, Titan IV citrate agar and IEP gels. Disposable paper wicks or sponges allow use with electrophoresis media supported by a rigid, flat base up to 6" x 9". A heat shield is included to protect gels from heat buildup. Unique interlock system breaks electrical circuit when chamber lid is removed.
Paper and Sponge Wicks
| Cat. No. | Item |
| 5081 | Zip Zone Chamber Wicks, 500/pkg |
| 9014 | Sponge Wicks - Short, 4 short sponges/pkg |
| 9015 | Sponge Wicks - Long, 2 long sponges/pkg |
Zip Zone Chamber Wicks are disposable paper wicks used with the Titan III cellulose acetate system to provide buffer flow during electrophoresis. Sponge Wicks provide sufficient buffer flow during agar and agarose techniques such as IEP and citrate hemoglobin electrophoresis.
Chamber Cooling Device and Coolant Sponges
| Cat. No. | Item |
| 5045 | Coolant Sponges, 10/pkg |
Cooling Device is used in TITAN GEL or Zip Zone chamber to reduce heat during TITAN GEL High Resolution Protein electrophoresis. The device is refrigerated, then placed in chamber immediately prior to electrophoresis. Gels are placed directly on cooling device and wicked to outside buffer troughs. Coolant sponges keep chambers cool during separations where heat can cause denaturation. Store sponges in freezer, then place in center troughs of chamber during electrophoresis.
Blotters
TITAN® Blotter Pads
| Cat. No. | Item |
| 5034 | Blotter Pads, 76 x 102 mm, 100/box |
| 5037 | Blotter Pads, 89 x 108 mm, 100/box |
| 5098 | Blotter Pads, 203 x 241 mm, 25/pkg |
These soft, absorbent paper blotters are used to remove excess buffer or reagent from cellulose acetate membranes or agarose gels.
Note: Blotters for Helena agarose procedures are packaged in each gel kit.
Electrophoresis Buffers
Electra®, Citrate and Supre-Heme® Buffers
| Cat. No. | Item |
| 5016 | Electra® B1 Buffer Barbital/Sodium Barbital/Na Azide, |
| 10 x 13.1 g | |
| 5121 | Citrate Buffer Sodium Citrate/Citric Acid, 10 x 15.5 g |
| 5802 | Supre-Heme® Buffer Tris-EDTA/Boric Acid, 10 x 14.6 g |
Note: Appropriate buffers for SPIFE, REP and TITAN GEL Agarose kits are packaged as part of each kit.
Stains, Clearing Solutions
| Cat. No. | Item |
| 3048 | Amido Black Protein Stain, 1 x 2.5 g |
Used as an alternative to Coomassie Brilliant Blue in the TITAN GEL High Res Protein procedure and for other procedures requiring a good basic protein stain. Dissolve stain in 450 mL methanol, 450 mL deionized water and 100 mL glacial acetic acid.
| Cat. No. | Item |
| 3049 | TITAN GEL IEP Stain, 1 x 10 g |
| Cat. No. | Item |
| 5322 | Lipoprotein Stain, 1 x 2.8 g |
| Cat. No. | Item |
| 5036 | o-Dianisidine, 1 x 2 g |
| Cat. No. | Item |
| 5041 | o-Tolidine, 1 x 2 g |
| Cat. No. | Item |
| 5526 | Ponceau S Stain, 1 x 105 g |
| Cat. No. | Item |
| 5005 | Clear Aid, 1 x 250 mL |
| Cat. No. | Item |
| 4950 | PermaClear, 1 x 1 L |
Buffer and Stain Accessories
Bufferizer
| Cat. No. | Item |
| 5093 | Bufferizer, 1/pkg |
Bufferizer is used to uniformly soak cellulose acetate membranes prior to electrophoresis. To use, place up to 12 dry membranes in a carrying rack; set rack in Bufferizer bottom; place Bufferizer top over bottom; pour buffer into top. Buffer gradually drips through a small hole in the top, allowing a uniform liquid front to move up the dry membranes. Eliminates air bubbles caused by uneven soaking.
Staining Sets and Carrying Racks
| Cat. No. | Item |
| 5122 | 1000 mL Staining Set, 7 trays with lids, 1 rack |
| 5110 | Titan III Carrying Rack, 1/pkg |
Staining set consists of 7 plastic trays with lids and a carrying rack for staining and soaking of cellulose acetate membranes. Trays hold 1000 mL of liquid. Carrying rack is designed to hold up to twelve 60 x 76 mm or 94 x 76 mm Titan III cellulose acetate membranes. One rack is included with the staining set; rack is also available separately.
Staining Dishes
| Cat. No. | Item |
| 4061 | Staining Dish, 1/pkg |
| 1362 | REP Gel Staining Dish, 10/pkg |
The Lipoprotein Staining Dish is 175 x 102 x 50 mm and may be used for cellulose acetate and agarose lipoprotein electrophoresis procedures. The REP Gel Staining Dish is 216 x 168 x 19 mm and may be used for REP procedures requiring staining.
Incubation Chamber and Development Weight
Incubation Chamber
| Cat. No. | Item |
| 4062 | TITAN GEL Isoenzyme Incubation Chamber, 1/pkg |
The TITAN GEL Isoenzyme Incubation Chamber is 171 x 89 x 6 mm and holds one gel. It maintains adequate humidity during the incubation process when running TITAN GEL CK and LD isoenzyme and ImmunoFix procedures.
Development Weight
| Cat. No. | Item |
| 5014 | Development Weight, 102 x 108 mm, 1/pkg |
The Development Weight can be used to press agarose gels. The weight also provides a constant surface temperature for incubating cellulose acetate membranes. Membranes are sandwiched between a prewarmed weight and a glass slide, then placed in incubator.
Sample Application Devices
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Applicators Super Z, Super Z-12, Super CK, and Macro Z applicators are used with Titan III cellulose acetate. Tips are photo-etched stainless steel and independently suspended to eliminate application artifacts. The only pressure exerted on the cellulose acetate is the weight of the tip. Super Z and Z-12 applicators hold 0.25 μL of sample; Super CK applicator holds 0.5 μL. Super Z and Super CK applicators apply 8 samples simultaneously, Super Z-12 applies 12 samples. Macro Z applicator, available in 4-sample or 6-sample size, provides a wider electrophoresis pattern. Use Zip Zone Prep (Cat. No. 5090) to clean tip. |
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| Applicator | Applicator Kit* | Applicator | Sample Well Plate | Aligning Base | Replacement Tips |
| System | 1/pkg | 2/pkg | 1/pkg | 2/pkg | |
| Cat. No. | Cat. No. | Cat. No. | Cat. No. | Cat. No. | |
| Super Z | 4088 | 4084 | 4085 | 4086 | 4087 |
| Super Z-12 | 4093 | 4090 | 4096 | 4094 | 4087 |
| Super CK | 4094 | ||||
| Macro Z (4) | 4099 | 4101 | 4086 | ||
| Macro Z (6) | 4094 |
*Includes applicator, 2 sample well plates, aligning base and a bottle of Zip Zone Prep.
Zip Zone® and Titan® IV Applicator System
| Cat. No. | Item |
| 4084 | Zip Zone Applicator, 1/pkg |
| 4081 | Zip Zone Sample Well Plate, 1/pkg |
| 4082 | Zip Zone Aligning Base, 1/pkg |
| 4083 | Titan IV Aligning Base, 1/pkg |
| 5090 | Zip Zone Prep, 1 x 60 mL |
Zip Zone Applicator System is used with Titan IV Citrate Agar Hemoglobin Electrophoresis. Plastic tips apply samples uniformly without cutting agar. Titan IV Aligning Base allows two off-center applications for a total of 16 samples. Use Zip Zone Prep to clean applicator tips.
5 μL
Microdispenser
| Cat. No. | Item |
| 6008 | 5 μL Microdispenser, 1 dispenser, 100 bores |
| 6010 | Glass Bores, 100/pkg |
Microdispenser features precision stainless steel plunger and disposable glass bores. Aluminum "collars" allow dispensing from 1 to 5 μL, in 1 μL increments.
Dialamatic
Microdispenser
| Cat. No. | Item |
| 6210 | 10 μL Microdispenser, 1 mL increments |
| 6211 | 10 μL Dialamatic Tubes, 100/pkg |
| 6225 | 25 μL Microdispenser, 1 mL increments |
| 6226 | 25 μL Dialamatic Tubes, 100/pkg |
| 6250 | 50 μL Microdispenser, 2 mL increments |
| 6251 | 50 μL Dialamatic Tubes, 100/pkg |
| 6276 | 100 μL Microdispenser, 100/pkg |
Dialamatic Microdispensers feature: sample preset, stainless steel plunger (10 mL unit), replaceable plunger tips (25, 50, and 100 mL units), precision glass disposable bores, ± 1% volumetric tolerance.
| Cat. No. | Item | Description |
| 6228 | Parts Kit for 25 μL Microdispenser | 3 Teflon tips, 1 plunger,
2 o-rings, |
| 1 collet, 1 coil spring & 1 spring guide | ||
| 6253 | Parts Kit for 50 μL Microdispenser | 3 Teflon tips, 1 plunger,
2 o-rings, |
| 1 collet, 1 coil spring & 1 spring guide |
REP and REP 3 Accessories
REP Preparation solutions, REP Prepper
| Cat. No. | Item | Description |
| 1574 | SUREprep | 3 x 250 mL |
| 3100 | REP Prep Solution | 1 x 250 mL |
SUREprep is a priming agent for REP applicator tips, ensuring precise sample application. It's also a cleaning agent for the tips. REP Prep is a wetting agent applied to the floor of the electrophoresis chamber to promote complete contact between gel and chamber floor. The dispenser bottles make it easy to apply SUREprep and deionized water; packaged to provide one dispenser each for SUREprep and deionized water. REP Prepper is a device for air-drying the pre-cut sample wells in REP gels. Sample wells must be free of moisture when samples are applied in order to maintain precise sample absorption.
REP and REP 3 Accessories and Replacement Parts
| Cat. No. | Item | Description |
| 1044 | DZA Replacement Lamp for REP (not for REP 3) | 1/pkg |
| 1117 | UV Replacement Lamp | 1/pkg |
| 1361 | REP/SPIFE Contact Sheets | 5/pkg |
| 1362 | REP Gel Staining Dish | 10/pkg |
| 1364 | Replacement REP Pipette Assembly | |
| 1577 | Replacement Tips for REP | 17/pkg |
- (Not interchangeable between REP and REP 3)
REP and REP 3 Electrodes, Reagent Spreaders
| Cat. No. | Item | Description |
| 1349 | REP IEF (Isoelectric Focusing) Electrodes & Adapters | 3 electrodes, 2 adapters |
| 1360 | REP Electrodes | 2/pkg |
| 1363 | REP Glass Rods (for ALP, LD, HDL) | 2/pkg |
| 3387 | Applicator Blade Weights (for SPIFE, REP) | 3/pkg |
| 3706 | Reagent Spreaders for SPIFE 3000 and REP 3 | 2/pkg |
| 3704 | SPIFE/REP 3 IFE Electrodes & Adapters | 3/electrodes, 2 adapters |
| 3709 | SPIFE/REP 3 Replacement Electrodes | 2/pkg |
Printer Supplies and Diskettes for REP Analyzers
| Cat. No. | Item | Description |
| 1424 | Toner Cartridge for Brother 1240 or 1440 Laser Printer | 1/pkg |
| 1425 | Drum for Brother 1240 Laser Printer | 1/pkg |
| 1465 | Toner Cartridge for Brother HL-630 Laser Printer 1/pkg | 1/pkg |
Miscellaneous Electrophoresis Supplies
GelGrip™
From the diagnostic leader in agarose electrophoresis products comes GelGrip,™ an advanced polymeric support media for most gel electrophoresis applications.GelGrip is a clear, flexible support for cast gels that ensures the physical integrity of the media through casting, electrophoresis, drying and imaging. Agarose covalently bonds to the casting side of GelGrip while the other side is inert for ease of use with conventional glass plate casting methods. GelGrip can be used in most staining and destaining techniques, including acid and alcohol based stains and destains. GelGrip is UV-opaque below 300 nm, suitable for photography and densitometry.
Try GelGrip. It offers superior agarose gel immobilization at a lower cost than other media.
| Cat. No. | Item |
| 2734 | GelGrip (85 x 100mm), 100 sheets/pkg |
| 2745 | GelGrip (110 x 125mm), 100 sheets/pkg |
| 2746 | GelGrip (100 x 150mm), 100 sheets/pkg |
| 2748 | GelGrip (110 x 205mm), 100 sheets/pkg |
| 2749 | GelGrip (160 x 180mm), 100 sheets/pkg |
| 2759 | GelGrip (125 x 245mm), 100 sheets/pkg |
| 2761 | GelGrip (124 x 258mm), 100 sheets/pkg |
| 2740 | GelGrip (102mm x 16.5m roll) |
| 2750 | GelGrip (127mm x 16.5m roll) |
| 2760 | GelGrip (152mm x 16.5m roll) |
| 2780 | GelGrip (203mm x 16.5m roll) |
Micropreparation Dish
| Cat. No. | Item |
| 8003 | Micropreparation Dish, 1/pkg |
The micropreparation dish is convenient for preparing samples for hemoglobin procedures and fetal lung maturity testing by TLC. It is constructed of chemically resistant nylon. Eight separate sample areas accommodate volumes of 1 mL or less. The conical shape assures easy access to entire sample solution.
Titan® and TITAN GEL® Plastic Envelopes
| Cat. No. | Item |
| 5052 | Titan Envelopes, 121 x 64 mm, 200/pkg |
| 5053 | Titan Envelopes, 115 x 102 mm, 100/pkg |
| 5054 | TITAN GEL Envelopes, 86 x 156 mm, 100/pkg |
These clear plastic envelopes, with convenient overlapping sleeve, enable you to store completed electrophoresis membranes and gels without scratching or tearing. They afford ideal protection for both completed TITAN III cellulose acetate and TITAN GEL agarose gels. Cat. No. 5054 can be used with Iso·Dot and Multi-Slot gels.
Identification Labels
| Cat. No. | Item |
| 5006 | Titan Envelopes, 121 x 64 mm, 200/pkg |
Titan ID Labels are convenient, pressure-sensitive identification labels for Titan III membranes. Simply press label onto edge of membrane or Titan Plastic Envelope to provide a paper surface on which to identify the sample.
Helena® Marker
| Cat. No. | Item |
| 5000 | Helena Marker, 1/pkg |
The Helena Marker can be used to write identifying information on the support side of cellulose acetate membranes and agarose gels. Will not wash off in most solvents.
Helena® Glue Stick
| Cat. No. | Item |
| 5002 | Glue Stick, 1/pkg |
The Glue Stick is an easy-to-use adhesive for mounting densitometric tracings on the report forms.
AVES Electrophoresis Chamber and Sponges
| Cat. No. | Item |
| 1299 | AVES Electrophoresis Chamber, 1 pkg |
CliniScan 2, CliniScan 3, EDC Accessories, Parts and Service
| Cat. No. | Item |
| 1032 | Neutral Density Densitometer Control |
| 1044 | DZA Replacement Lamp for REP, EDC, CliniScan 2 |
| 1047 | Optical Density Step Tablet, 1/pkg |
| 1117 | U.V. Replacement Lamp for REP, EDC, CliniScan 2 |
Neutral Density Densitometer Controls
| Cat. No. | Item |
| 1031 | Neutral Density Densitometer Control for QuickScan |
| 1032 | Neutral Density Densitometer Control |
The NDDC can be used to verify densitometer accuracy. The NDDC provides a test scan with known values for 5 fractions. To use, scan the NDDC as you would an electrophoresis pattern. The values obtained should correlate to the printed values for each fraction.
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Neutral Density Densitometer Controls |
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| Cat. No. | Item | |
| 1047 | Optical Density Step Tablet (10-step) | |
| The Optical Density Step Tablet is a valuable tool for checking the linearity of a densitometer and for estimating the optical density of electrophoresis and TLC fractions. | ||